MCB3020L General Microbiology Laboratory
Activity #3
Section: U01
Lab Title: Further Isolation of Bacterial Sample: Characterization, Gram Staining and Re-streaking.
Date: 02/15/20222
Student Name: Thalia Ramos Gigato.
Student ID: 6141550
CLASS ACTIVITY WORKSHEET
Table 1: Isolation of chosen colony #1
Note: Some of this information is obtained from your previous class activity.
|
Sample |
Picture |
Description |
|
Isolated streak on nutrient agar plate #1 |
|
Colony Morphology: Size: small Shape: punctiform Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque Other: They aren’t as much spread out as the plates from last week. Does this match the previous colony chosen from serial dilution plates? Why or why not? Yes, they have the same colony morphology. Is it pure? Why or why not? They look about the same. |
|
Gram stains of tertiary streak |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: Gram negative, thin peptidoglycan. Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, because the bacteria look the same. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because the still have rod shape and are gram negative. Is it pure? Why or why not? Yes, because they have the same characteristics. |
|
Gram stains of tertiary streak |
100x objective |
|
|
Gram stains of tertiary streak |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: gram negative, thin peptidoglycan Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, because they have the same chape, color, and arrangement. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because the bacteria is negative and present rod shape. Is it pure? Why or why not? Yes, because they look the same. |
|
Gram stains of tertiary streak |
100x objective |
|
|
Gram stains of the Tail Region |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: gram negative, thin peptidoglycan Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, because the bacteria have the same shape, color, and arrangement. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because it shows the same results. Is it pure? Why or why not? Yes, because they share the same characteristics. |
|
Gram stains of the Tail Region |
100x objective |
|
|
Gram stains of the Tail Region |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: gram negative, thin peptidoglycan Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, they look the same. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because the bacteria have the same characteristics. Is it pure? Why or why not? Yes, because they have same color, arrangement, and shape so it’s considered a pure colony. |
|
Gram stains of the Tail Region |
100x objective |
Table 2: Isolation of chosen colony #2
Note: Use the guidelines above to help replicate the table for nutrient agar plate #2
|
Sample |
Picture |
Description |
|
Isolated streak on nutrient agar plate #2 |
|
Colony Morphology: Size: small Shape: punctiform Color: creamy white Margins: even Elevation: flat Surface: smooth Opacity: opaque Other: The colonies are close Does this match the previous colony chosen from serial dilution plates? Why or why not? Yes, they have the same colony morphology. Is it pure? Why or why not? Yes, because they have the same characteristics. |
|
Gram stains of tertiary streak |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: gram negative, thin peptidoglycan. Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, because they show the same characteristics. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because the colony has the same characteristics. Is it pure? Why or why not? Yes, because the bacteria have the same shape, arrangement, and color. |
|
Gram stains of tertiary streak |
100x objective |
|
|
Gram stains of tertiary streak |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: gram negative, thin peptidoglycan Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, because the bacteria still have the same characteristics. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because by the characteristics we can assume is the exact same bacteria. Is it pure? Why or why not? Yes, because it presents the same shape, arrangement, and color. |
|
Gram stains of tertiary streak |
100x objective |
|
|
Gram stains of the Tail Region |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: gram negative, thin peptidoglycan. Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, because they have the same colony morphology. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because by the characteristics of the bacteria they are the same. Is it pure? Why or why not? Yes, because the bacteria show the same shape, arrangement, and color. |
|
Gram stains of the Tail Region |
100x objective |
|
|
Gram stains of the Tail Region |
40x objective |
Cellular Morphology: Size: small Shape: rods Arrangement: cluster Color: pink Staining characteristics: gram negative, thin peptidoglycan Potential errors? None Do the 40x and 100x pictures match? Why or why not? Yes, because the have the same characteristics. Does this Gram stain result match the previous colony (obtained from serial dilution)? Why or why not? Yes, because by the characteristics both show they are the same. Is it pure? Why or why not? Yes, because the have the same shape, arrangement, and color. |
|
Gram stains of the Tail Region |
100x objective |
1
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